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KMID : 0613820140240010039
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Journal of Life Science
2014 Volume.24 No. 1 p.39 ~ p.45
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Ghost Vaccine Prepared from Strong Virulent Salmonella typhimurium Does not Improve Immune Responses of BALB/c Mice
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Ha Yeon-Jo
Kim Tae-Wan
Kim Seung-Tae
Gal Sang-Wan
Kim Sam-Woong
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Abstract
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Salmonella typhimurium MMP13 and S. typhimurium ×8554 were derived from weak JOL401 and strong ×3339 virulent strains. Heat-labile subunit B (LT-B) was used as an adjuvant to increase the effectiveness of the vaccine. Plasmid pMMP184 carrying a ghost cassette was transformed into MMP13 and ×8554 to produce the ghost, and the prepared ghost cells were administered into the muscles of BALB/c mice. In the absence of the adjuvant, the total IgG content showed a tendency to increase contrary to the original virulent strength. In contrast, in the presence of the adjuvant, the strain that originated from the strong virulent showed a tendency to promote the immune more than that of weak virulent strain. However, the final concentration of total IgG was similar between the compared groups, indicating that the originated virulent strength does not affect a specific immune. Other elements of the immunoglobulins IgG1, IgG2a, and sIgAs did not show a specific trend. The results of Salmonella challenge showed a similar tendency to regardless of the originated virulence. Taken together, the results suggest that the Salmonella ghost cells promoted the immune system of BALB/c mice, irrespective of the virulence applied to create the ghost cells.
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KEYWORD
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Ghost vaccine, immunoglobulin, protection, S. typhimurium, virulence
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